non-linear regression to an exponential growth equation  (GraphPad Software Inc)

Journal: Parasites & Vectors

Article Title: Differential susceptibility of bovine caruncular and trophoblast cell lines to infection with high and low virulence isolates of Neospora caninum

doi: 10.1186/s13071-017-2409-9

Figure Lengend Snippet: Proliferation kinetics over time and tachyzoite yield at 58 hpi. Graphs ( a and b ) represent the average number of tachyzoites for each time-point for all individual experiments with an R 2 > 0.95, except for BCEC-1 cells infected by Nc-Spain1H, which showed a non-exponential growth pattern. Error bars indicate the SD. Representative images ( c ) show the proliferation kinetics over time of Nc-Spain7 and Nc-Spain1H isolates in F3 and BCEC-1 cultures. The bar graph ( d ) represents the tachyzoite yield at 58 hpi for Nc-Spain7 and Nc-Spain1H in F3 and BCEC-1 cells. Each column and error bar represents the mean and the SD of 4 replicates from 2 independent assays. The TY 58h was fifteen times higher in F3 cells than in BCEC-1 cells infected with Nc-Spain7, and ten times higher in F3 cells infected with Nc-Spain1H. Statistical differences were found in the TY 58h between isolates in F3 cells, with the TY 58h of Nc-Spain7 significantly higher than for Nc-Spain1H ( P < 0.0001). * represents significant differences between isolates. Scale-bars : c , 10 μm

Article Snippet: The T d was determined by applying non-linear regression analysis and an exponential growth equation using GraphPad (San Diego, CA, USA).

Techniques: Infection